Description
Principle of the assay: This assay employs a two-site sandwich ELISA to quantitative TSR1 in Human serum, plasma. An antibody specific for TSR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for TSR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RT-PCR ELISA detected very high expression in all specific adult brain regions examined. Intermediate expression was detected in heart, whole adult brain, liver, skeletal muscle, kidney, testis, ovary, and spinal cord, and in fetal brain and liver. Little to no expression was detected in pancreas and spleen. Tsr1, encodes a 788-amino acid protein with an N-terminal and C-terminal domain separated by a central acidic domain. The tripartite organization shares similarity to Bms1, but Tsr1 lacks the P-loop required for GTP binding and an obvious nuclear localization signal.Tsr1 is required for assembly of a transportor maturation-competent particle or is specifically required for transport of 43S pre-ribosomal particles, but not 60S ribosome precursors, from the nucleus to the cytosol